Use the various tools and strategies utilized in the construction and production of
recombinant DNA molecules in vitro and in vivo. Learn the various techniques utilized for the introduction of recombinant DNA
molecules in bacteria, yeast and mammalian cells.
Elucidate the steps involved in the genetic engineering from amplification of DNA
molecules to cloning of molecules, and screening strategies for clone identification.
. Understand the importance of high capacity vectors, plasmids and the various steps
involved in genomic library preparation to understand complex genomes.
Differentiate between different gene mapping methods, analysis of gene expression by
various methods, and techniques used for introduction of mutations
- Teacher: Dr. Bhavyashree U. Udyawar